Abstract
In viewing of the potential of lactic acid bacterial (LAB) in enhancing antioxidant activity of food products, this work explored the effectiveness of LAB fermented black tea samples in alleviating H2O2–induced oxidative stress in human colonocytes. The antioxidant capacity of tea samples was evaluated in terms of cyto-protectiveness, mitochondria membrane potential (Δψm)-stabilizing activity, ROS-inhibitory effect and antioxidant enzyme-modulating activity. The effect on oxidative DNA damage and repair was studied in CCD 841 by comet assay. Results showed that the protective effect of tea pre-treatment was more pronounced in normal cells (CCD 841) than in carcinomas (Caco-2), and fermented samples were invariably more effective. Higher cell viability and Δψm were maintained and ROS production was markedly inhibited with tea pre-treatment. The fermented tea samples also remarkably stimulated DNA repair, resulting in less strand breaks and oxidative lesions. Our study implied that LAB fermentation may be an efficient way to enhance the antioxidative effectiveness of black tea flavonoid-enriched foods.
Quercetin-3-O-glucoside (QG) and kaempferol-3-O-glucoside (KG) 88 (Herbpurify Co., Ltd, Chengdu, China)
